This product is a box of 10 vials.
TB-500 FAQ:
BPC-157 FAQ:
BPC-157 and TB-500 Blend
BPC-157 and TB-500 (Thymosin Beta-4) are peptides often studied for their potential to promote tissue repair and healing. Both have unique but complementary ways of working that, when combined, may provide enhanced benefits. BPC-157 is a stable 15-amino-acid peptide derived from a protective protein in the stomach lining. Preclinical studies show it speeds up healing in muscles, tendons, and ligaments by encouraging the growth of new blood vessels and regulating growth factors. Similarly, TB-500 is a fragment of the natural Thymosin Beta-4 protein, which plays a major role in cell movement, tissue regeneration, and reducing inflammation.
The possible synergy between these peptides comes from their overlapping but different paths in tissue repair. BPC-157 is great at improving blood flow and cutting inflammation, while TB-500 helps cells move to the injury site and supports repair at the cellular level. Together, they might speed up healing by tackling various parts of recovery, especially in sports injuries or long-term conditions where regeneration is limited. Early animal studies suggest this combo could lead to quicker recovery and stronger repaired tissues.
Chemical Diagrams:
Compound 1: TB-500
Sequence: Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser
Molecular formula: C212H350N56O78S
Molar Mass: 4963.4408
CAS number: 77591-33-4
PubChem: CID 16132341
Compound 2: BPC-157
Sequence: Gly- Glu-Pro-Pro-Pro-Gly-Lys-Pro-Ala-Asp-Asp-Ala-Gly-Leu-Val
Molecular Formula: C62H98N16O22
Molecular Weight: 1419.556 g/mol
PubChem CID: 108101
Biochemistry
TB500 is a synthetic peptide derived from thymosin beta-4 (TB-4), a naturally occurring protein in the human body known for its anti-inflammatory and tissue-repairing properties. Like TB-4, TB500 binds to actin and plays a role in gene regulation. It has shown benefits across various systems, including cardiovascular health, muscle regeneration, immune modulation, and central nervous system function. TB500 has even been linked to promoting hair growth and easing some effects of aging.
TB500 operates through two primary mechanisms:
- Actin Sequestration and Cell Motility Regulation:Within cells, TB500 binds to and sequesters actin, a key component of the cytoskeleton. This interaction is crucial for regulating cell movement, growth, and division. By enhancing cell motility, TB500 supports faster wound healing, improved immune cell trafficking, reduced inflammation, and increased angiogenesis (blood vessel formation).
- Gene Expression Modulation ("Moonlighting" Function): Beyond its structural role, TB500 also influences gene expression independent of actin binding. It modulates the expression of genes involved in nitric oxide (NO) production, angiogenesis, and cellular proliferation—paralleling several downstream effects observed with BPC-157. Through this regulatory function, TB500 affects cytokine secretion and modulates multiple signaling pathways associated with the inflammatory response. Notably, it downregulates pathways such as NF-κB and Toll-like receptor signaling, thereby suppressing pro-inflammatory cytokines like TNF-α and IL-1 receptor-associated kinases.
In addition to controlling inflammation, TB500 activates several tissue repair pathways, including PI3K/Akt/eNOS, Notch, and angiopoietin-1/Tie signaling. One of its most studied actions involves modulation of the TGF-β pathway, which plays a central role in reducing fibrosis (scarring)—making TB500 particularly valuable in the context of chronic injury and tissue regeneration.
As an aside, there is research to show that TB500 influences Wnt signaling. This has been shown to promote the formation of hair follicles and boost hair growth at the level of DNA expression patterns. The diagram below offers an overview of the complex and wide-ranging influences of TB500.
BPC-157 is a synthetic peptide made of 15 amino acids. It comes from a naturally occurring compound in the body known as Body Protective Compound (BPC). Originally isolated from human gastric juice, BPC has shown strong anti-inflammatory and wound-healing properties. In animal studies, BPC-157 has shown potential tissue regeneration benefits across various systems, including the gastrointestinal tract, liver, pancreas, ligaments, muscles, tendons, cornea, heart, brain, and nerves.
While the exact way BPC-157 works is not fully clear, several ideas have emerged. It is not yet known if the peptide acts by binding to cell surface receptors or by entering cells and directly influencing genetic activity. In truth, BPC-157 may work through both methods.
Research has consistently shown that BPC-157 greatly affects nitric oxide (NO) signaling—particularly through its regulation of endothelial nitric oxide synthase (eNOS). This is believed to explain many of its therapeutic effects because NO promotes the movement of vascular endothelial cells that are important in tissue repair and the growth of new blood vessels. There is recent evidence showing that BPC-157 promotes the phosphorylation of Src, Cav-1, and eNOS. It also reduces the binding interaction between Cav-1 and eNOS, an essential step in eNOS activation and NO production.
BPC-157 is known for its rapid absorption and spread throughout the body when taken by mouth or injected. Within 10 minutes of injection, the peptide is detectable throughout the body, including the kidneys, liver, stomach wall, thymus, reproductive organs, and spleen. Peak concentrations in tissues are typically reached about one hour after administration and gradually decline over the following 48 hours. The highest levels are usually found in the kidney, liver, thymus, and spleen, with moderate distribution in the lungs, muscles, brain, and skin.
Many of BPC-157's effects are thought to occur through changes in gene expression patterns. Again, the mechanism for this is not fully understood, but the peptide has been shown to clearly alter the expression patterns of:
• Egr,
• Nos (especially eNos),
• Srf,
• Vegr,
• Plcγ, and
• Kras.
These genes control the synthesis of a number of factors that affect cells of blood vessels and the immune system. BPC-157 has been shown to alter fundamental properties of cells like migration, adhesion, thrombosis, and inflammatory responses. These alterations result in changes to cell behavior that help regulate inflammation and improve tissue healing. Additional research shows that expression levels of each gene are increased or decreased based on the timeframe following administration of BPC-157. In other words, the length of time that BPC-157 is in the system is important in determining which genes are activated. This suggests that BPC-157 works through a complex regulatory mechanism that has fine control over a wide array of genes and their expression.
Potential BPC-157 and TB-500 Synergy in Wound Repair
Research shows that BPC-157 and TB-500, which both stimulate wound healing through different biochemical paths, may have enhanced effects when used together.
Cell Migration
Successful wound healing relies on fibroblasts, which manage the production of extracellular matrix, as well as immune system cells. For these cells to work, they must move to the injury site. This movement, called migration, depends heavily on the protein actin. Both BPC-157 and TB-500 play roles in regulating actin. BPC-157 boosts actin production at the gene level. TB-500, as an actin-binding protein, helps gather actin in spots where it's most needed to form filaments that enable cell movement. Together, BPC-157 and TB-500 team up to increase the amount and function of actin, speeding up how quickly fibroblasts and immune cells reach injured areas.
Growth Hormone
Both TB-500 and BPC-157 interact with growth hormone during healing. BPC-157 increases the number of growth hormone receptors on fibroblasts, extending these cells' lifespan and improving their ability to promote soft tissue regeneration. With TB-500 added, the extra receptors won't be wasted because the fibroblasts will have plenty of actin to use during their longer lifespan. Combining TB-500, BPC-157, collagen, and a growth hormone booster is a reliable way to speed up wound healing and could one day become the standard treatment.
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