{"id":142557,"date":"2020-12-03T17:59:35","date_gmt":"2020-12-03T09:59:35","guid":{"rendered":"https:\/\/iasuperpharma.is\/ph\/?post_type=product&#038;p=142557"},"modified":"2025-12-03T01:44:20","modified_gmt":"2025-12-02T17:44:20","slug":"pnc","status":"publish","type":"product","link":"https:\/\/iasuperpharma.is\/ph\/shop\/injectables\/peptides\/pnc\/","title":{"rendered":"PNC"},"content":{"rendered":"<h5>PNC<\/h5>\n<div class=\"packaging\">Packaging: Single vial containing lyophilized product.<\/div>\n<table class=\"ingredient-chart\">\n<tbody>\n<tr>\n<th colspan=\"3\">EACH VIAL CONTAINS:<\/th>\n<\/tr>\n<tr>\n<td>PNC<\/td>\n<td>BP<\/td>\n<td>5mg<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n\n","protected":false},"excerpt":{"rendered":"<p>The anti-cancer activity and mechanism of PNC-27, which carries a p53 aa12-26-leader sequence connected on its carboxyl terminal end to a trans-membrane-penetrating sequence or membrane residency peptide (MRP), was studied against p53-null K562 leukemia cells. Murine leukocytes were used as a non-cancer cell control. Necrosis was determined by measuring the lactate dehydrogenase (LDH) release and apoptosis was determined by the detection of Caspases 3 and 7. Membrane colocalization of PNC-27 with HDM-2 was analyzed microscopically using fluorescently labeled antibodies against HDM-2 and PNC-27 peptides.<\/p>\n<p>Results found that K562 cells strongly express HDM-2 protein in their membranes and that PNC-27 co-localizes with this protein in the membranes of these cells. PNC-27, but not the negative control peptide PNC-29, is selectively cytotoxic to K562 cells, inducing nearly 100 percent cell killing with LDH release. In contrast, this peptide had no effect on the lymphocyte control cells.<\/p>\n<p>The results suggest that HDM-2 is expressed in the membranes of non-solid tissue tumor cells in addition to the membranes of solid tissue tumor cells. Since K-562 cells appear to be in the stem cell family, the results suggest that early developing tumor cells also express HDM-2 protein in their membranes. Since PNC-27 induces necrosis of K-562 leukemia cells and co-localizes with HDM-2 in the tumor cell membrane as an early event, we conclude that the association of PNC-27 with HDM-2 in the cancer cell membrane results in trans-membrane pore formation which results in cancer cell death, as previously discovered in a number of different solid tissue tumor cells. Since K562 cells lack p53 expression, these effects of PNC-27 on this leukemia cell line occur by a p53-independent pathway.<\/p>\n","protected":false},"featured_media":525085,"template":"","meta":{"_acf_changed":false,"inline_featured_image":false,"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"default","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","ast-disable-related-posts":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"product_brand":[],"product_cat":[1309,133],"product_tag":[],"class_list":{"0":"post-142557","1":"product","2":"type-product","3":"status-publish","4":"has-post-thumbnail","6":"product_cat-injectables","7":"product_cat-peptides","8":"product_shipping_class-peptides","9":"desktop-align-left","10":"tablet-align-left","11":"mobile-align-left","13":"first","14":"instock","15":"shipping-taxable","16":"product-type-simple"},"bundled_by":[],"bundle_stock_status":"instock","bundle_stock_quantity":null,"bundle_virtual":false,"bundle_layout":"","bundle_add_to_cart_form_location":"","bundle_editable_in_cart":false,"bundle_sold_individually_context":"","bundle_item_grouping":"","bundle_min_size":"","bundle_max_size":"","bundle_price":[],"bundled_items":[],"bundle_sell_ids":[],"acf":[],"_links":{"self":[{"href":"https:\/\/iasuperpharma.is\/ph\/wp-json\/wp\/v2\/product\/142557","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/iasuperpharma.is\/ph\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/iasuperpharma.is\/ph\/wp-json\/wp\/v2\/types\/product"}],"version-history":[{"count":7,"href":"https:\/\/iasuperpharma.is\/ph\/wp-json\/wp\/v2\/product\/142557\/revisions"}],"predecessor-version":[{"id":779425,"href":"https:\/\/iasuperpharma.is\/ph\/wp-json\/wp\/v2\/product\/142557\/revisions\/779425"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/iasuperpharma.is\/ph\/wp-json\/wp\/v2\/media\/525085"}],"wp:attachment":[{"href":"https:\/\/iasuperpharma.is\/ph\/wp-json\/wp\/v2\/media?parent=142557"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/iasuperpharma.is\/ph\/wp-json\/wp\/v2\/product_brand?post=142557"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/iasuperpharma.is\/ph\/wp-json\/wp\/v2\/product_cat?post=142557"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/iasuperpharma.is\/ph\/wp-json\/wp\/v2\/product_tag?post=142557"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}